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snapf tag  (New England Biolabs)


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    Structured Review

    New England Biolabs snapf tag
    Snapf Tag, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 347 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/snapf+tag/SNAP-Cell+TMR-STAR/bio_rxiv__2025__02__26__640415-147-3-7
    Average 96 stars, based on 347 article reviews
    snapf tag - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Distinct Clinical Phenotypes in KIF1A-Associated Neurological Disorders Result from Different Amino Acid Substitutions at the Same Residue in KIF1A
    Article Snippet: .. To label the SNAPf-tag, SNAP-Cell® TMR-Star ligand (New England Biolabs, # S9105S) was added to the resin to a final concentration of 10 μM before elution, and the resin was incubated at RT for 20 minutes. ..

    Article Title: Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibited and Activated
    Article Snippet: .. If fluorescent labeling of the SNAPf tag was required, SNAP-Cell TMR-Star (New England Biolabs) was suspended to 1 mM in 100% DMSO (Sigma-Aldrich), added to the dynein coated beads to a final concentration of 10 μM and incubated on a roller for 1-2 hr before continuing the purification. ..

    Incubation:

    Article Title: Distinct Clinical Phenotypes in KIF1A-Associated Neurological Disorders Result from Different Amino Acid Substitutions at the Same Residue in KIF1A
    Article Snippet: .. To label the SNAPf-tag, SNAP-Cell® TMR-Star ligand (New England Biolabs, # S9105S) was added to the resin to a final concentration of 10 μM before elution, and the resin was incubated at RT for 20 minutes. ..

    Article Title: Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibited and Activated
    Article Snippet: .. If fluorescent labeling of the SNAPf tag was required, SNAP-Cell TMR-Star (New England Biolabs) was suspended to 1 mM in 100% DMSO (Sigma-Aldrich), added to the dynein coated beads to a final concentration of 10 μM and incubated on a roller for 1-2 hr before continuing the purification. ..

    Ligation:

    Article Title: In vitro reconstitution of a highly processive recombinant human dynein complex
    Article Snippet: .. Ligation-independent infusion (Clontech) cloning was used to seamlessly insert a SNAPf tag (New England Biolabs) to generate pDyn1. .. Genes for IC2C, LIC2, Tctex1, LC8 and Robl1 were assembled into pIDC (Vijayachandran et al , ), with each expression cassette separated by 30 bp linkers consisting of random sequence and a unique restriction site, to generate pDyn2. pDyn1 and pDyn2 were fused using an in vitro Cre reaction (New England Biolabs) to form pDyn3.

    Cloning:

    Article Title: In vitro reconstitution of a highly processive recombinant human dynein complex
    Article Snippet: .. Ligation-independent infusion (Clontech) cloning was used to seamlessly insert a SNAPf tag (New England Biolabs) to generate pDyn1. .. Genes for IC2C, LIC2, Tctex1, LC8 and Robl1 were assembled into pIDC (Vijayachandran et al , ), with each expression cassette separated by 30 bp linkers consisting of random sequence and a unique restriction site, to generate pDyn2. pDyn1 and pDyn2 were fused using an in vitro Cre reaction (New England Biolabs) to form pDyn3.

    FLAG-tag:

    Article Title: Autoinhibition and cooperative activation mechanisms of cytoplasmic dynein.
    Article Snippet: Cytoplasmic dynein is a two-headed microtubule-based motor responsible for diverse intracellular movements, including minus-end-directed transport of organelles.. The motility of cargo transporters is regulated according to the presence or absence of cargo; however, it remains unclear how cytoplasmic dynein achieves such regulation.. Here, using a recombinant and native dynein complex in vitro, we show that lone, single dynein molecules are in an autoinhibited state, in which the two motor heads are stacked together.

    Labeling:

    Article Title: Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibited and Activated
    Article Snippet: .. If fluorescent labeling of the SNAPf tag was required, SNAP-Cell TMR-Star (New England Biolabs) was suspended to 1 mM in 100% DMSO (Sigma-Aldrich), added to the dynein coated beads to a final concentration of 10 μM and incubated on a roller for 1-2 hr before continuing the purification. ..

    Purification:

    Article Title: Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibited and Activated
    Article Snippet: .. If fluorescent labeling of the SNAPf tag was required, SNAP-Cell TMR-Star (New England Biolabs) was suspended to 1 mM in 100% DMSO (Sigma-Aldrich), added to the dynein coated beads to a final concentration of 10 μM and incubated on a roller for 1-2 hr before continuing the purification. ..



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    Workflow for expression of pCS2+/SNAPf- and Halo-tagged constructs in Xenopus embryos pCS2+/SNAPf- and Halo-vectors that were codon optimized for Xenopus can be acquired from Addgene. The sequence of interest is cloned into the multiple cloning site (MCS), located upstream/downstream of the SNAPf/Halo-tag. Tagged constructs are in vitro transcribed to generate mRNA that is microinjected into 2–4 cell stage Xenopus embryos.

    Journal: STAR Protocols

    Article Title: SNAP- and Halo-tagging and dye introduction protocol for live microscopy in Xenopus embryos

    doi: 10.1016/j.xpro.2022.101622

    Figure Lengend Snippet: Workflow for expression of pCS2+/SNAPf- and Halo-tagged constructs in Xenopus embryos pCS2+/SNAPf- and Halo-vectors that were codon optimized for Xenopus can be acquired from Addgene. The sequence of interest is cloned into the multiple cloning site (MCS), located upstream/downstream of the SNAPf/Halo-tag. Tagged constructs are in vitro transcribed to generate mRNA that is microinjected into 2–4 cell stage Xenopus embryos.

    Article Snippet: Workflow for expression of pCS2+/SNAPf- and Halo-tagged constructs in Xenopus embryos pCS2+/SNAPf- and Halo-vectors that were codon optimized for Xenopus can be acquired from Addgene.

    Techniques: Expressing, Construct, Sequencing, Clone Assay, In Vitro

    Journal: STAR Protocols

    Article Title: SNAP- and Halo-tagging and dye introduction protocol for live microscopy in Xenopus embryos

    doi: 10.1016/j.xpro.2022.101622

    Figure Lengend Snippet:

    Article Snippet: Workflow for expression of pCS2+/SNAPf- and Halo-tagged constructs in Xenopus embryos pCS2+/SNAPf- and Halo-vectors that were codon optimized for Xenopus can be acquired from Addgene.

    Techniques: Recombinant, Software, Microscopy